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Detection of the Existence of Anthocyanin Structural Gene by Morpho-molecular Analysis in Capsicum spp.


Anthocyanins are pigments widespread in nature and responsible for red, blue, and purple
colors in many plant tissues including fruits, stems, leaves, flowers and roots. Anthocyanin are
accumulated at different levels in plants and have several biological functions, such as attracting
pollinators, preventing plant photo-oxidative damage, and play important role in plant defense. In
chilli peppers (Capsicum spp.), anthocyanin are commonly detected have correlation with
resistance level of plants in response to pathogen attack such as antracnose.
Anthocyanin is attributed to purple pigmentation in other Capsicum plant parts. This
pigmentation content is highly detected in ornamental peppers. Therefore, to increase the level of
anthocyanin in an improved variety of pepper by transferring anthocyanin biosynthesis genes to
edible peppers in a breeding program.
Six enzymes are generally involved in anthocyanin biosynthesis, such as CHS, CHI, F3H, DFR,
ANS, and UFGT. Chalcone synthase (CHS) is the first enzymatic step of biosynthetic pathway.
Chalcone is isomerized by chalcone isomerase (CHI) to naringenin. Flavanone 3-hydroxylase
(F3H) converts naringen into dihydrokaempferol. The dihydroflavonols are subsequently
converted to anthocyanins by action of three enzymes, that are DFR, ANS, and UFGT. Study the
existence of anthocyanin structural gene as the basic method for selection in a breeding program
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Tantri Swandari - Personal Name
Panjisakti Basunanda - Personal Name
Adi Cahya Kurniawan - Personal Name
Aziz-Purwantoro - Personal Name
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Indonesia
Artikel Dosen
2014
Artikel Dosen
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