Yohana Theresia Maria Astuti; " />
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GIBBERELLIC ACID SYNTHESIS IN THE DEVELOPING SEEDS OF COCOA


An experiment was carried out to determine gibberellic acid synthesis in the developing cacao seeds. Two cacao clones namely KW163 and KW165 were used. Several plants for each clone were selected and hand pollination was conducted before anthesis. Samples of healthy seeds were taken at 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 weeks following hand pollination. Gibberellic acid content was determined by HPLC (High Performance Liquid Chromatography). Molecular study was carried out to know expression of TcGA20ox like gene on seeds aged seven weeks. The results showed that, in seeds aged 1-10 weeks, the giberellic acid content in developing seeds of clone KW163 fluctuated from about 1068.10 ng.g -1 fw at week 1 to 181.92 ng.g -1 fw at week 10 and the giberellin content in developing seeds of clone KW165 fluctuated from about 1762.49 ng.g -1 fw at week 1 to 714.54 ng.g -1 fw at week 10. TcGA20ox like gene that could be found in seeds indicated that there was gibberellic acid synthesis in the developing seeds.

Keywords: Cocoa seed, synthesis, gibberellic acid, TcGA20ox like gene
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Corresponding author: astuti_maria2000@yahoo.com
Yohana Theresia Maria Astuti - Personal Name
Kumala Dewi - Personal Name
Santosa - Personal Name
A. Adi Prawoto - Personal Name
ICGAI, Yogyakarta, INDONESIA, November 12-14, 2013
ISBN: 978-979-18768-3-4
NONE
Field Trip held on 11 November 2013 Seminar held on 12 – 13 November 2013 in Faculty of Agriculture, UPN “Veteran” Yogyakarta Indonesia
Text
UPN VETERAN YOGYAKARTA
2013
yogyakarta
Proceedings International Conference on Green Agro
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